Green Qpcr Primers

The primers are meticulously designed using origene s proprietary primer design algorithm developed from 10 000 qpcr experiments.

Green qpcr primers.

Refer to the mircury lna rt kit handbook for details. To comply with miqe they have been developed using sophisticated bioinformatics tools and validated in silico to avoid off target amplification 1. Design sybr green qpcr primers experimental protocol oct 31 2013 recommendations. The mircury lna mirna pcr assays and panels have been validated using the mircury sybr green master mix.

1 find the ncbi gene. Sybr green for qpcr. Qstar qpcr primer pairs are designed for sybr green based real time qpcr. Sybr green i is a commonly used fluorescent dye that binds double stranded dna molecules by intercalating between the dna bases.

Use of other reagents for qpcr will affect the results. Our primer collection covers the entire human and mouse genomes. It is used in quantitative pcr because the fluorescence can be measured at the end of each amplification cycle to determine relatively or absolutely how much dna has been amplified. The azuraquant green fast qpcr mix is a ready to use 2x master mix for use in real time quantitative pcr assays in which intercalating dye based detection provides the option of a post amplification melt profile.

Azuraquant green and probe fast qpcr master mixes for real time pcr real time pcr primer enzymes.